ISOLATION AND CHARACTERIZATION OF PISANG TONGKAT LANGIT (Musa troglodytarum L.) ACS1 AND ACO1 GENE EXPRESSION DURING FRUIT RIPENING PROCESS

Pisang tongkat langit (Musa troglodytarum L.) is a banana cultivar originated from Eastern Indonesia. Previous studies showed that ACS1 and ACO1 are genes play an important role in ethylene biosynthesis. However, characterization analysis of M. have not been reported. This study aims to isolate characterize the gene expression profile during fruit ripening process. Gene fragment isolation was performed using PCR based method. Results isolated genomic fragments 400 bp 500 bp. cDNA 200 300 Analysis BLAST NCBI had high homology with 1818 Musa acuminata AAA Group (GenBank: GQ396304.1), while highest 1593 AJ223232.1). one intron two exons, introns three exons. Homology conserved region similar acuminata.